-- Contents.- Special Methods of Isolation and Purification.- Enzymes du Métabolisme du Soufre.- A. Enzymes d''activation et de transfert du sulfate.- I. Enzymes d''activation du sulfate.- 1. Mesure de l''activité enzymatique.- a) Système complet d''activation.- b) ATP-sulfurylase -- Robbins et Lipmann (1958a).- c) ADP-sulfurylase -- Robbins et Lipmann (1958a).- d) APS-kinase -- Robbins et Lipmann (1958a).- 2. Préparation de l''ATP-sulfurylase, de l''ADP-sulfurylase et de l''APS-kinase.- II. Enzymes de transfert du sulfate.- 1. Mesure de l''activité de la phénol-sulfokinase.- 2. Préparation de la phénol-sulfokinase -- Gregory et Lipmann (1957) . ..- B. Systèmes réducteurs du sulfate.- 1. Mesure de l''activité sulfato-réductrice.- 2. Préparation du système réducteur du sulfate, Hilz, Kittler et Knape (1959).- C. Sulfatases.- 1. Arylsulfatases.- Préparation des arylsulfatases.- 2. Chondrosulfatases.- 3. Myrosulfatases.- 4. Glucosulfatases.- 5. Stéroïdesulfatases.- 6. Cholinesulfatase.- D. Enzymes du métabolisme du thiosulfate, du sulfite et du sulfure.- I. Enzymes du métabolisme du thiosulfate.- 1. Thiosulfate-réductase.- 2. Rhodanèse.- II. Enzymes du métabolisme du sulfite.- 1. Formation du sulfite par désulfination.- 2. Sulfite-réductase.- 3. Sulfite-oxydase.- III. Enzymes du métabolisme du sulfure.- 1. Formation d''hydrogène sulfuré à partir de la cystéine.- 2. Sérine-sulfhydrase.- 3. Oxydation du sulfure.- Bibliographie.- Enzymes of Phosphate Metabolism.- I. Special Features of Enzyme Isolation and Purification.- 1. Choice of Starting Material.- 2. Conventional versus Modern Methods of Enzyme Purification.- a) Automatic Device for Ammonium Sulfate Addition.- b) Dialysis of Enzyme Solutions.- 3. Stability of Enzymes on Dilution.- II. Assay of Phosphatase Activity.- 1. Colorimetric Estimation of Phosphorus.- 2. Aspects of Enzyme Assay in Plants and Microorganisms.- III. Intracellular Localization of Enzymes by the Technique of Differential Centrifugation.- 1. Nuclear Fraction.- 2. Plastid Fraction.- 3. Mitochondrial Fraction.- 4. Cell Wall Fraction.- 5. Association of Enzymes with Larger Particles.- 6. Isolation of Plant Protoplasts.- 7. Localization of Phosphatases.- IV. Phosphatases Acting on Hexose Phosphates.- 1. The Hydrolysis of Glucose-1-Phosphate.- 2. C1 Diphosphatases.- a) Fructose Diphosphatase.- b) Fructose Diphosphatase of Animal Tissue.- c) Fructose Diphosphatase of Plants.- d) Neutral Fructose Diphosphatase.- e) Acidic Fructose Diphosphatase.- f) Acid C1 Diphosphatase of Escherichia coli.- g) Role of Alkaline Fructose Diphosphatase in Green Plants.- 3. Sedoheptulose-1,7 -Diphosphatase.- 4. Glycerate-2,3-Diphosphatase.- V. Enzymes Acting on Condensed Phosphates.- 1. Inorganic Pyrophosphatases.- a) Inorganic Pyrophosphatase of Microorganisms.- b) Inorganic Pyrophosphatases of Animal Tissue.- 2. Tripolyphosphatases.- 3. Tetrapolyphosphatases.- 4. Trimetaphosphatases.- 5. Tetrametaphosphatases.- VI. Enzymes Acting on Polyphosphates.- Type I Enzyme: Polyphosphate Depolymerases.- Type II Enzyme: Polyphosphatases.- a) Type I.- b) Type II.- c) Type III.- Type III Enzyme: Enzymes Transfering Phosphate Groups from Polyphosphates to Suitable Acceptors.- Polyphosphate-ADP-phosphotransferase of Escherichia coli.- VII. General Phosphomonoesterases.- 1. Acid Phosphatases.- 2. Alkaline Phosphatases.- a) Alkaline Phosphatases of Microorganisms.- VIII. Phosphoprotein Phosphatase.- IX. Ortho Phosphoserine Phosphatase.- X. Phosphatase Acting on Nucleotides and Nucleic Acids.- 1. Nucleotide Pyrophosphatases.- a) Nucleotide Pyrophosphatase of Potato.- b) Nucleotide Pyrophosphatase of Tobacco.- 2. 5''-Nucleotidases.- a) 5''-Nucleotidase of Clostridium stricklandii.- 3. Ribonucleases.- a) Ribonucleases of Plants.- b) Endonucleases from Microorganisms.- c) Phosphodiesterases Acting on Polynucleotides.- References.- Enzymes of Inorganic Nitrogen Metabolism.- A. Nitrate Reductase.- I. Preparation.- 1. Fungi and Higher Plants.- 2. Bacteria.- a) Methods of extraction.- b) Fractionation and Stability.- II. Measurement of Activity.- 1. Assay Methods.- 2. Sources of Error.- III. Electron Donors and Co-Factors.- 1. Fungi and Higher Plants.- a) Pyridine nucleotides and other electron donors.- b) Flavins.- c) Metal and Anion Requirements.- 2. Bacteria.- IV. Properties and Mechanisms.- 1. Fungi and Higher Plants.- 2. Bacteria.- V. Physiological Factors.- 1. Fungi and Higher Plants.- 2. Bacteria.- B. Other Nitrate Reduction and Nitro Reductase Enzymes.- 1. Aldehyde and Xanthine Oxidases of Animal Origin.- 2. Aldehyde Oxidase of Potato.- 3. Reduction of Aromatic Nitro-Compounds.- C. Nitrite, Nitric Oxide, Hyponitrite and Hydroxylamine Reductases and Related Enzymes.- I. Preparation.- 1. Fungi and Higher Plants.- 2. Bacteria.- II. Fractionation and Stability.- III. Measurement of Activity.- 1. Interference and Errors.- 2. Assay Methods.- a) Use of CONWAY Methods for NH3 Determinations.- b) Hydroxylamine.- c) Oximes, YAMAFUJI and AKITA (1952).- d) Hyponitrite.- e) Assay Methods Using Reduced Benzyl Viologen and Other Dyes under Anaerobic Conditions.- f) Manometric Assay of Nitrite Reductase.- g) Manometric Assay of Nitric Oxide Reductase.- 3. Preparation of Hyponitrite as a Substrate.- IV. Electron Donors and Co-Factors and Inhibitors.- 1. Fungi and Higher Plants.- a) Nitrite Reductase.- b) Hyponitrite Reductase.- c) Hydroxylamine Reductase.- 2. Bacteria.- a) Nitrite Reductase.- b) Nitric Oxide Reductase.- c) Hydroxylamine Reductase.- V. Properties and Mechanisms.- 1. Fungi and Higher Plants.- 2. Bacteria.- a) Nitrite Reductase.- b) Nitric Oxide Reductase.- c) Hydroxylamine Reductase.- VI. Physiological Factors and Other Features.- 1. Fungi and Higher Plants.- a) Nitrite Reductase.- b) Hydroxylamine Reductase.- D. Glutamic Dehydrogenase.- E. Oxidation and Other Reactions of Ammonia, Hydroxylamine and Nitrite.- I. Fungi and Higher Plants.- Hydroxylamine "Oxidase" of Higher Plants.- II. Bacteria (Enzymes of Nitrification).- 1. Preparation.- 2. Measurement of Activity.- 3. Cofactors.- 4. Properties and Mechanisms.- Reversal of NH2OH reductase "Ammonia dehydrogenase" artefact.- Literature.- Enzymes of Vitamin Metabolism.- A. Enzymes Concerned with Biosynthesis.- 1. Thiamine.- a) General Preparation of Enzyme Fractions.- b) Pyrimidine Kinase.- c) Thiazole Kinase.- d) Thiamine Phosphate Synthetase..- e) Thiamine Phosphatase.- 2. Riboflavin.- 3. Nicotinic Acid.- a) Tryptophan Pyrrolase.- b) Formylase (Kynurenine Formamidase).- c) Kynurenine Hydroxylase.- d) Kynureninase.- 4. Folic Acid.- 5. Pantothenic Acid.- Pantothenate Synthetase.- B. Activating Enzymes.- 1. Thiamine.- a) Thiamine Pyrophosphokinase.- b) Thiamine Pyrophosphate Kinase.- 2. Riboflavin.- a) Flavokinase.- b) FAD Pyrophosphorylase.- 3. Pyridoxal (Vitamin B6).- Pyridoxal Phosphokinase.- 4. Nicotinic Acid.- a) PRPP-Nicotinic Acid Transferase.- b) DPN-Pyrophosphorylase.- c) DPN-Synthetase.- d) DPN-Kinase.- 5. Folic Acid.- a) Folic Acid Reductase.- b) Dihydrofolic Acid Reductase.- 6. Pantothenic Acid.- a) Pantothenate Kinase.- b) Coupling Enzyme.- c) Phosphopantothenylcysteine Decarboxylase.- d) Dephospho-CoA Pyrophosphorylase.- e) Phosphotransacetylase.- f) Dephospho-CoA Kinase.- C. Degrading Enzymes.- 1. Thiamine.- a) Thiaminase.- b) Thiamine Phosphatase.- c) Thiamine Pyrophosphatase.- 2. Riboflavin.- a) FAD-ase (Nucleotide Pyrophosphatase).- b) FMN-Phosphatase.- 3. Nicotinic Acid.- a) Nucleotide Pyrophosphatase.- b) DPN-ase (Pyridine Transglycosidase).- c) NMN-Phosphatase.- 4. Folic Acid.- 5. Pantothenic Acid.- a) "CoA-3''-Nucleotidase".- b) "CoA Pyrophosphatase".- c) "CoA-Peptidase".- 6. Biotin.- d-Biotin Oxidase.- 7. Inositol.- Phytase.- 8. Carotene.- Carotene Oxidase (Lipoxidase).- References.- Enzyme des L-Ascorbinsäure-Stoflwechsels.- I. Enzyme bei der Biosynthese der L-Ascorbinsäure.- 1. Indirekte Umwandlung.- a) Aldolkondensation.- b) Acyloin-Reaktion.- 2. Direkte Umwandlung.- a) L-Galaktonsäure-?-laktondehydrogenase.- b) Enzympräparat nach Mapson U. Isherwood (1958).- c) Enzympräparat nach Nakatani.- d) L-Gulonsäuredehydrogenase nach Ishikawa U. Noguchi (1957).- e) Herstellung der gereinigten Laktonase I.- II. L-Ascorbinsäure oxydierende Enzymsysteme.- 1. Peroxydase.- 2. Cytochrom c -- Cytochromoxydase.- 3. Polyphenoloxydase.- 4. Laccase.- 5. Ascorbinsäureoxydase.- a) Methoden der Kupferbestimmung.- b) Eigenschaften der AS-Oxydase.- c) Gewinnung der Ascorbinsäure-Oxydase.- d) Aktivitätsbestimmungen der AS-Oxydase.- e) Vorkommen der AS-Oxydase.- 6. Andere Ascorbinsäure oxydierende Enzyme.- III. L-Ascorbinsäure reduzierende Enzymsysteme.- 1. Herstellung des gereinigten Enzympräparates nach Janecke U. Ragab.- 2. Darstellung der L-Dehydroascorbinsäure.- 3. Verbreitung der DAS-Reduktase.- Literatur.- Enzymes Involved in the Synthesis and Breakdown of Indoleacetic Acid.- I. General Methods.- Separations of IAA from its Precursors in the Reaction Mixture.- II. Enzymes Involved in the Synthesis of IAA.- 1. Enzymes Involved in the Conversion of TTP to IAA.- a) Distribution.- b) Pathways for the Conversion of TTP to IAA.- c) Conversion of IPyA to IAc and IAA.- d) Preparation and Properties of Some Enzymes Converting TTP to IAA.- 2. Enzymes Involved in the Conversion of IAc to IAA.- Preparation of the Enzym es.- 3. Enzymes Involved in the Conversion of TNH2 to IAc and IAA.- 4. Enzymes Involved in the Conversion of IAN to IAA.- III. Enzymes Involved in the Breakdown of IAA.- 1. General Properties of the IAA Oxidation Reaction.- 2. Preparation and Properties of some IAA Oxidases.- a) Lupin Enzyme.- b) Pea Enzyme.- c) Wheat Leaf Enzyme.- d) Pineapple Enzyme.- e) Omphalia Enzyme.- References.- Enzymes of Aromatic Biosynthesis.- I. Enzymes of Aromatic Biosynthesis from Non-Aromatic Compounds in Microorganisms.- a) 2-Keto-3-Deoxy-D-Araboheptonic Acid-7-Phosphate (KDHP) Synthetase.- b) The Enzyme Converting KDHP to Dehydroquinate.- c) Dehydroshikimic (DHS) Dehydrase.- d) 5-Dehydroquinase